› 6fa3

X-ray diffraction
1.82Å resolution

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTPase KRas, N-terminally processed Chains: B, C, D, E, F
Molecule details ›
Chains: B, C, D, E, F
Length: 172 amino acids
Theoretical weight: 19.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-167; Coverage: 88%)
  • Best match: P01116-2 (Residues: 1-168)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 19.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-167; Coverage: 88%)
  • Best match: P01116-2 (Residues: 1-168)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 6 x MG
Ligand GNP 6 x GNP
Ligand D1Z 4 x D1Z
Ligand DMS 1 x DMS
1 modified residue:
Ligand CSO 5 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 63.39Å b: 118.48Å c: 156.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.207
Expression system: Escherichia coli

Quick links

Citations

25 review citations

RAS-targeted therapies: is the undruggable drugged?
Moore et al. (2020)
24 more

5 mentions without citation

RAS Nanoclusters: Dynamic Signaling Platforms Amenable to Therapeutic Intervention.
Van et al. (2021)
4 more