› 6fbt

X-ray diffraction
2.5Å resolution

The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa in complex with the reaction product NAG-anhNAMpentapeptide

Released:
DOI: 10.2210/pdb6fbt/pdb
PDB entry 6fbt coloured by chain, front view.
PDB entry 6fbt coloured by chain, side view.
PDB entry 6fbt coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt of Pseudomonas aeruginosa.
Lee M, Batuecas MT, Tomoshige S, Domínguez-Gil T, Mahasenan KV, Dik DA, Hesek D, Millán C, Usón I, Lastochkin E, Hermoso JA, Mobashery S
Proc Natl Acad Sci U S A 115 4393-4398 (2018)
PMID: 29632171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable soluble lytic transglycosylase Chain: A
Molecule details ›
Chain: A
Length: 610 amino acids
Theoretical weight: 69.97 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HZI6 (Residues: 30-639; Coverage: 99%)
Gene name: PA3020
Sequence domains:
NAG-anhNAMpentapeptide Chain: E
Molecule details ›
Chain: E
Length: 5 amino acids
Theoretical weight: 533 Da
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand PO4 5 x PO4
Ligand NAG 1 x NAG
Ligand AH0 1 x AH0
3 modified residues:
Ligand DGL 1 x DGL
Ligand API 1 x API
Ligand DAL 2 x DAL

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P63
Unit cell:
a: 165.526Å b: 165.526Å c: 54.733Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
2 more

3 mentions without citation

Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt of Pseudomonas aeruginosa.
Lee et al. (2018)
2 more