6fe8

Electron Microscopy
3.7Å resolution

Cryo-EM structure of the core Centromere Binding Factor 3 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152823 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Centromere DNA-binding protein complex CBF3 subunit B Chains: A, B
Molecule details ›
Chains: A, B
Length: 584 amino acids
Theoretical weight: 68.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40969 (Residues: 47-608; Coverage: 92%)
Gene names: CBF3, CBF3B, CEP3, CSL1, YM8520.17C, YMR168C
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit B
Suppressor of kinetochore protein 1 Chain: C
Molecule details ›
Chain: C
Length: 197 amino acids
Theoretical weight: 22.56 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P52286 (Residues: 2-194; Coverage: 100%)
Gene names: CBF3D, D9798.14, SKP1, YDR328C
Sequence domains:
Centromere DNA-binding protein complex CBF3 subunit C Chain: D
Molecule details ›
Chain: D
Length: 519 amino acids
Theoretical weight: 60.9 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P35203 (Residues: 2-478; Coverage: 100%)
Gene names: CBF3C, CTF13, YM6543.01, YM9582.19, YMR094W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-4241
Expression system: Saccharomyces cerevisiae