› 6fki

Electron Microscopy
4.3Å resolution

Chloroplast F1Fo conformation 3

Released:
DOI: 10.2210/pdb6fki/pdb
PDB entry 6fki coloured by chain, front view.
PDB entry 6fki coloured by chain, side view.
PDB entry 6fki coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Structure, mechanism, and regulation of the chloroplast ATP synthase.
Hahn A, Vonck J, Mills DJ, Meier T, Kühlbrandt W
Science 360 (2018)
PMID: 29748256
Related structures: EMD-4272

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
PDBe Complex ID:
PDB-CPX-133606 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
ATP synthase subunit a, chloroplastic Chain: a
Molecule details ›
Chain: a
Length: 247 amino acids
Theoretical weight: 27.1 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P06451 (Residues: 1-247; Coverage: 100%)
Gene name: atpI
Sequence domains: ATP synthase A chain
ATP synthase subunit b, chloroplastic Chain: b
Molecule details ›
Chain: b
Length: 184 amino acids
Theoretical weight: 21.01 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P06453 (Residues: 1-184; Coverage: 100%)
Gene name: atpF
Sequence domains: ATP synthase B/B' CF(0)
ATP synthase delta chain, chloroplastic Chain: d
Molecule details ›
Chain: d
Length: 257 amino acids
Theoretical weight: 27.71 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P11402 (Residues: 1-257; Coverage: 100%)
Gene name: ATPD
Sequence domains: ATP synthase delta (OSCP) subunit
ATP synthase subunit b', chloroplastic Chain: p
Molecule details ›
Chain: p
Length: 222 amino acids
Theoretical weight: 24.49 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P31853 (Residues: 1-222; Coverage: 100%)
Gene names: ATPF2, ATPG
Sequence domains: ATP synthase B/B' CF(0)
ATP synthase epsilon chain, chloroplastic Chain: e
Molecule details ›
Chain: e
Length: 134 amino acids
Theoretical weight: 14.72 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P00833 (Residues: 1-134; Coverage: 100%)
Gene name: atpE
Sequence domains:
ATP synthase gamma chain, chloroplastic Chain: g
Molecule details ›
Chain: g
Length: 364 amino acids
Theoretical weight: 40.12 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P05435 (Residues: 1-364; Coverage: 100%)
Gene name: ATPC
Sequence domains: ATP synthase
ATP synthase subunit c, chloroplastic Chains: G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 81 amino acids
Theoretical weight: 7.98 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P69447 (Residues: 1-81; Coverage: 100%)
Gene name: atpH
Sequence domains: ATP synthase subunit C
ATP synthase subunit alpha, chloroplastic Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 507 amino acids
Theoretical weight: 55.51 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P06450 (Residues: 1-507; Coverage: 100%)
Gene name: atpA
Sequence domains:
ATP synthase subunit beta, chloroplastic Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 498 amino acids
Theoretical weight: 53.8 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P00825 (Residues: 1-498; Coverage: 100%)
Gene name: atpB
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 3 x ATP
Ligand MG 5 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.3Å
Relevant EMDB volumes: EMD-4272

Quick links

Citations

36 review citations

The assembly, regulation and function of the mitochondrial respiratory chain.
Vercellino et al. (2022)
35 more

8 mentions without citation

ATP synthase FOF1 structure, function, and structure-based drug design.
Vlasov et al. (2022)
7 more