› 6fn3

X-ray diffraction
1.9Å resolution

X-ray structure of animal-like Cryptochrome from Chlamydomonas reinhardtii

Released:
DOI: 10.2210/pdb6fn3/pdb
PDB entry 6fn3 coloured by chain, front view.
PDB entry 6fn3 coloured by chain, side view.
PDB entry 6fn3 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.
Franz S, Ignatz E, Wenzel S, Zielosko H, Putu EPGN, Maestre-Reyna M, Tsai MD, Yamamoto J, Mittag M, Essen LO
OpenAccess logo Nucleic Acids Res 46 8010-8022 (2018)
PMID: 30032195

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cryptochrome photoreceptor Chain: A
Molecule details ›
Chain: A
Length: 509 amino acids
Theoretical weight: 57.81 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8J8W0 (Residues: 1-496; Coverage: 83%)
Gene name: CHLRE_06g278251v5
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MES 1 x MES
Ligand FAD 1 x FAD
Ligand GOL 11 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P212121
Unit cell:
a: 151.8Å b: 50.13Å c: 65.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.206
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity.
Falciatore et al. (2020)
3 more

2 mentions without citation

Structure of the bifunctional cryptochrome aCRY from Chlamydomonas reinhardtii.
Franz et al. (2018)
1 more