› 6fny

X-ray diffraction
2.79Å resolution

CRYSTAL STRUCTURE OF A CHOLINE SULFATASE FROM SINORHIZOBIUM MELLILOTI

Released:
DOI: 10.2210/pdb6fny/pdb
PDB entry 6fny coloured by chain, front view.
PDB entry 6fny coloured by chain, side view.
PDB entry 6fny coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Primary publication:
Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester.
van Loo B, Schober M, Valkov E, Heberlein M, Bornberg-Bauer E, Faber K, Hyvönen M, Hollfelder F
OpenAccess logo J Mol Biol 430 1004-1023 (2018)
PMID: 29458126

Function and Biology Details

Reaction catalysed: 
Choline sulfate + H(2)O = choline + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline-sulfatase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 515 amino acids
Theoretical weight: 58.81 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli
UniProt:
  • Canonical: O69787 (Residues: 2-512; Coverage: 100%)
Gene names: R00949, SMc00127, betC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
1 modified residue:
Ligand DDZ 8 x DDZ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 119.556Å b: 163.248Å c: 177.625Å
α: 90° β: 94.4° γ: 90°
R-values:
R R work R free
0.207 0.205 0.252
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Sated by a Zero-Calorie Sweetener: Wastewater Bacteria Can Feed on Acesulfame.
Kleinsteuber et al. (2019)
3 more

2 mentions without citation

Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester.
van Loo et al. (2018)
1 more