› 6fuz

X-ray diffraction
2.7Å resolution

Crystal structure of the TPR domain of KLC1 in complex with the C-terminal peptide of JIP1

Released:
DOI: 10.2210/pdb6fuz/pdb
PDB entry 6fuz coloured by chain, front view.
PDB entry 6fuz coloured by chain, side view.
PDB entry 6fuz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors.
Pernigo S, Chegkazi MS, Yip YY, Treacy C, Glorani G, Hansen K, Politis A, Bui S, Dodding MP, Steiner RA
OpenAccess logo Elife 7 (2018)
PMID: 30320553

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-522593 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-Jun-amino-terminal kinase-interacting protein 1; Kinesin light chain 1 Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 37.3 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O88447 (Residues: 199-495; Coverage: 55%)
  • Canonical: Q9UQF2 (Residues: 701-711; Coverage: 2%)
Gene names: IB1, JIP1, Klc1, Kns2, MAPK8IP1, PRKM8IP
Sequence domains:
nanobody Chain: N
Molecule details ›
Chain: N
Length: 121 amino acids
Theoretical weight: 13.4 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 107.473Å b: 90.364Å c: 51.698Å
α: 90° β: 99.8° γ: 90°
R-values:
R R work R free
0.234 0.233 0.257
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

10 citation in other articles

Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.
Fukuda et al. (2021)
9 more

3 mentions without citation

Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors.
Pernigo et al. (2018)
2 more