› 6fvm

X-ray diffraction
1.63Å resolution

Mutant DNA polymerase sliding clamp from Escherichia coli with bound P7 peptide

Released:
DOI: 10.2210/pdb6fvm/pdb
PDB entry 6fvm coloured by chain, front view.
PDB entry 6fvm coloured by chain, side view.
PDB entry 6fvm coloured by chain, top view.
Source organisms:
Primary publication:
Interaction of a Model Peptide on Gram Negative and Gram Positive Bacterial Sliding Clamps.
André C, Martiel I, Wolff P, Landolfo M, Lorber B, Silva da Veiga C, Dejaegere A, Dumas P, Guichard G, Oliéric V, Wagner J, Burnouf DY
ACS Infect Dis 5 1022-1034 (2019)
PMID: 30912430

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-199623 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta sliding clamp Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 40.87 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
P7 peptide Chains: H, I
Molecule details ›
Chains: H, I
Length: 6 amino acids
Theoretical weight: 701 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand 1PE 9 x 1PE
Ligand GOL 1 x GOL
1 modified residue:
Ligand ALC 2 x ALC

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 34.336Å b: 78.653Å c: 80.182Å
α: 113.26° β: 97.82° γ: 95.97°
R-values:
R R work R free
0.182 0.18 0.219
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Modulators of protein-protein interactions as antimicrobial agents.
Kahan et al. (2021)
1 more