› 6g0i

X-ray diffraction
2Å resolution

Active Fe-PP1

Released:
DOI: 10.2210/pdb6g0i/pdb
PDB entry 6g0i coloured by chain, front view.
PDB entry 6g0i coloured by chain, side view.
PDB entry 6g0i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Effects of stably incorporated iron on protein phosphatase-1 structure and activity.
Salvi F, Trebacz M, Kokot T, Hoermann B, Rios P, Barabas O, Köhn M
OpenAccess logo FEBS Lett 592 4028-4038 (2018)
PMID: 30403291

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 1-330; Coverage: 100%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand FE 2 x FE
Ligand MN 2 x MN
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Unit cell:
a: 38.365Å b: 68.686Å c: 126.995Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.233
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A.
Hoermann et al. (2020)
4 more

2 mentions without citation

Towards Dissecting the Mechanism of Protein Phosphatase-1 Inhibition by Its C-Terminal Phosphorylation.
Salvi et al. (2021)
1 more