6g4w Citations

Visualizing late states of human 40S ribosomal subunit maturation.

Nature 558 249-253 (2018)
Related entries: 6g18, 6g4s, 6g51, 6g53, 6g5h, 6g5i

Cited: 72 times
EuropePMC logo PMID: 29875412

Abstract

The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.

Reviews - 6g4w mentioned but not cited (1)

  1. Ribosome biogenesis factors-from names to functions. Dörner K, Ruggeri C, Zemp I, Kutay U. EMBO J 42 e112699 (2023)

Articles - 6g4w mentioned but not cited (6)



Reviews citing this publication (11)

  1. Eukaryotic Ribosome Assembly. Baßler J, Hurt E. Annu Rev Biochem 88 281-306 (2019)
  2. Uncovering the assembly pathway of human ribosomes and its emerging links to disease. Bohnsack KE, Bohnsack MT. EMBO J 38 e100278 (2019)
  3. Ribosome Biogenesis in Plants: From Functional 45S Ribosomal DNA Organization to Ribosome Assembly Factors. Sáez-Vásquez J, Delseny M. Plant Cell 31 1945-1967 (2019)
  4. Pre-Ribosomal RNA Processing in Human Cells: From Mechanisms to Congenital Diseases. Aubert M, O'Donohue MF, Lebaron S, Gleizes PE. Biomolecules 8 E123 (2018)
  5. Maturation of pre-40S particles in yeast and humans. Cerezo E, Plisson-Chastang C, Henras AK, Lebaron S, Gleizes PE, O'Donohue MF, Romeo Y, Henry Y. Wiley Interdiscip Rev RNA 10 e1516 (2019)
  6. RNA folding and functions of RNA helicases in ribosome biogenesis. Mitterer V, Pertschy B. RNA Biol 19 781-810 (2022)
  7. The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches. Gerovac M, Vogel J, Smirnov A. Front Mol Biosci 8 661448 (2021)
  8. Quality control ensures fidelity in ribosome assembly and cellular health. Parker MD, Karbstein K. J Cell Biol 222 e202209115 (2023)
  9. The Rio1 protein kinases/ATPases: conserved regulators of growth, division, and genomic stability. Berto G, Ferreira-Cerca S, De Wulf P. Curr Genet 65 457-466 (2019)
  10. Genetics animates structure: leveraging genetic interactions to study the dynamics of ribosome biogenesis. Black JJ, Johnson AW. Curr Genet 67 729-738 (2021)
  11. A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Life. Jüttner M, Ferreira-Cerca S. Methods Mol Biol 2533 3-22 (2022)

Articles citing this publication (54)