Function and Biology Details
Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Synaptotagmin-1 and Botulinum neurotoxin type B (preferred)
PDBe Complex ID:
PDB-CPX-145487 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin B heavy chain
Molecule details ›
Chains: A, B
Length: 461 amino acids
Theoretical weight: 55.16 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 461 amino acids
Theoretical weight: 55.16 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P10844 (Residues: 857-1291; Coverage: 34%)
Sequence domains:
- Clostridium neurotoxin, N-terminal receptor binding
- Clostridium neurotoxin, C-terminal receptor binding
Synaptotagmin-1
Molecule details ›
Chains: 33, C
Length: 21 amino acids
Theoretical weight: 2.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 21 amino acids
Theoretical weight: 2.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: P21579 (Residues: 33-53; Coverage: 5%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
DIAMOND BEAMLINE I03
Spacegroup:
P21
Expression systems:
- Escherichia coli BL21(DE3)
- Not provided
