› 6gb8

X-ray diffraction
1.48Å resolution

Copper nitrite reductase from Achromobacter cycloclastes: small cell polymorph dataset 1

Released:
DOI: 10.2210/pdb6gb8/pdb
PDB entry 6gb8 coloured by chain, front view.
PDB entry 6gb8 coloured by chain, side view.
PDB entry 6gb8 coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Primary publication:
Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.
Ebrahim A, Appleby MV, Axford D, Beale J, Moreno-Chicano T, Sherrell DA, Strange RW, Hough MA, Owen RL
OpenAccess logo Acta Crystallogr D Struct Biol 75 151-159 (2019)
PMID: 30821704

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-540824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.06 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25006 (Residues: 39-378; Coverage: 100%)
Gene name: nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand NO2 1 x NO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P213
Unit cell:
a: 96.382Å b: 96.382Å c: 96.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Advances in methods for atomic resolution macromolecular structure determination.
Thompson et al. (2020)
1 more

1 mention without citation

Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.
Ebrahim et al. (2019)