› 6gcg

X-ray diffraction
1.8Å resolution

Copper nitrite reductase from Achromobacter cycloclastes: large polymorph dataset 15

Released:
DOI: 10.2210/pdb6gcg/pdb
PDB entry 6gcg coloured by chain, front view.
PDB entry 6gcg coloured by chain, side view.
PDB entry 6gcg coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Primary publication:
Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.
Ebrahim A, Appleby MV, Axford D, Beale J, Moreno-Chicano T, Sherrell DA, Strange RW, Hough MA, Owen RL
OpenAccess logo Acta Crystallogr D Struct Biol 75 151-159 (2019)
PMID: 30821704

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 40.82 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25006 (Residues: 1-378; Coverage: 100%)
Gene name: nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P213
Unit cell:
a: 97.989Å b: 97.989Å c: 97.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Advances in methods for atomic resolution macromolecular structure determination.
Thompson et al. (2020)
1 more

1 mention without citation

Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.
Ebrahim et al. (2019)