› 6grv

Solution NMR

Cadmium(II) form of full-length metallothionein from Pseudomonas fluorescens Q2-87 (PflQ2 MT)

Released:
DOI: 10.2210/pdb6grv/pdb
PDB entry 6grv coloured by chain, ensemble of 20 models, front view.
PDB entry 6grv coloured by chain, ensemble of 20 models, side view.
PDB entry 6grv coloured by chain, ensemble of 20 models, top view.
Source organism: Pseudomonas fluorescens Q2-87
Primary publication:
A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc.
Habjanič J, Zerbe O, Freisinger E
Metallomics 10 1415-1429 (2018)
PMID: 30191219

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallothionein Chain: A
Molecule details ›
Chain: A
Length: 81 amino acids
Theoretical weight: 8.82 KDa
Source organism: Pseudomonas fluorescens Q2-87
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J2EKT7 (Residues: 2-82; Coverage: 99%)
Gene names: MT, PflQ2_2045
Sequence domains: Prokaryotic metallothionein

Ligands and Environments

1 bound ligand:
Ligand CD 4 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Zinc homeostasis in Pseudomonas.
Ducret et al. (2023)
1 more