6gsm

Electron Microscopy
5.15Å resolution

Structure of a partial yeast 48S preinitiation complex in open conformation.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
PDBe Complex ID:
PDB-CPX-123452 (preferred)
Entry contents:
44 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (47 distinct):
Small ribosomal subunit protein uS2 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.14 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CN12 (Residues: 2-209; Coverage: 82%)
Gene names: KLLA0E16171g, RPS0
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein eS1 Chain: B
Molecule details ›
Chain: B
Length: 231 amino acids
Theoretical weight: 26.4 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CWD0 (Residues: 3-233; Coverage: 91%)
Gene names: KLLA0B05060g, RPS1
Sequence domains: Ribosomal S3Ae family
Small ribosomal subunit protein uS5 Chain: C
40S ribosomal protein S3 Chain: D
40S ribosomal protein S4 Chain: E
Small ribosomal subunit protein uS7 domain-containing protein Chain: F
Small ribosomal subunit protein eS6 Chain: G
40S ribosomal protein S7 Chain: H
40S ribosomal protein S8 Chain: I
KLLA0E23673p Chain: J
Plectin/eS10 N-terminal domain-containing protein Chain: K
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: L
40S ribosomal protein S12 Chain: M
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: N
Small ribosomal subunit protein uS11 Chain: O
Rps15 Chain: P
Small ribosomal subunit protein uS9 Chain: Q
KLLA0B01474p Chain: R
KLLA0B01562p Chain: S
KLLA0A07194p Chain: T
Small ribosomal subunit protein uS10 domain-containing protein Chain: U
Small ribosomal subunit protein eS21 Chain: V
Small ribosomal subunit protein uS8 Chain: W
40S ribosomal protein S23 Chain: X
40S ribosomal protein S24 Chain: Y
40S ribosomal protein S25 Chain: Z
40S ribosomal protein S26 Chain: a
40S ribosomal protein S27 Chain: b
Small ribosomal subunit protein eS28 Chain: c
Small ribosomal subunit protein uS14 Chain: d
40S ribosomal protein S30 Chain: e
Small ribosomal subunit protein eS31 Chain: f
Asc1 Chain: g
Small ribosomal subunit protein eS32A Chain: h
Eukaryotic translation initiation factor 1A Chain: i
Eukaryotic translation initiation factor 2 subunit alpha Chain: j
Eukaryotic translation initiation factor 2 subunit gamma Chain: k
Eukaryotic translation initiation factor 2 subunit beta Chain: l
Eukaryotic translation initiation factor eIF-1 Chain: m
Eukaryotic translation initiation factor 3 subunit A Chain: o
Eukaryotic translation initiation factor 3 subunit B Chain: p
Eukaryotic translation initiation factor 3 subunit C Chain: q
Eukaryotic translation initiation factor 3 subunit I Chain: s
Eukaryotic translation initiation factor 3 subunit G Chain: r
Met-tRNAi Chain: 1
18S Ribosomal RNA Chain: 2
mRNA (5'-R(P*AP*AP*U)-3') Chain: 3

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 5.15Å
Relevant EMDB volumes: EMD-0057
Expression systems:
  • Escherichia coli BL21(DE3)
  • Saccharomyces cerevisiae