› 6gvt

Solid-state NMR

Hybrid structure of the pRN1 helix bundle domain in complex with DNA and 2 ATP molecules

Released:
DOI: 10.2210/pdb6gvt/pdb
PDB entry 6gvt coloured by chain, ensemble of 20 models, front view.
PDB entry 6gvt coloured by chain, ensemble of 20 models, side view.
PDB entry 6gvt coloured by chain, ensemble of 20 models, top view.
Source organism: Sulfolobus islandicus
Primary publication:
A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template.
Boudet J, Devillier JC, Wiegand T, Salmon L, Meier BH, Lipps G, Allain FH
Cell 176 154-166.e13 (2019)
PMID: 30595448

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119972 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
SF3 helicase domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 115 amino acids
Theoretical weight: 13.53 KDa
Source organism: Sulfolobus islandicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q54324 (Residues: 256-370; Coverage: 13%)
Sequence domains: Primase helical domain
DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*CP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: Sulfolobus islandicus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 72%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Archaeal DNA Replication.
Greci et al. (2020)
1 more

4 mentions without citation

Sedimentation Yields Long-Term Stable Protein Samples as Shown by Solid-State NMR.
Wiegand et al. (2020)
3 more