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Structural basis of IMP3 RRM12 recognition of RNA.

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Jia M, Gut H, Chao JA
OpenAccess logo RNA 24 1659-1666 (2018)
Related entries: 6fq1, 6fqr

Cited: 15 times
EuropePMC logo PMID: 30135093

Abstract

The IMP family of RNA binding proteins, also named as insulin-like growth factor 2 (IGF2) mRNA-binding proteins (IGF2BPs), are highly conserved RNA regulators that are involved in many RNA processing stages, including mRNA stability, localization, and translation. There are three paralogs in the IMP family, IMP1-3, in mammals that all adopt the same domain arrangement with two RNA recognition motifs (RRM) in the N terminus and four KH domains in the C terminus. Here, we report the structure and biochemical characterization of IMP3 RRM12 and its complex with two short RNAs. These structures show that both RRM domains of IMP3 adopt the canonical RRM topology with two α-helices packed on an anti-parallel four stranded β-sheet. The spatial orientation of RRM1 to RRM2 is unique compared with other known tandem RRM structures. In the IMP3 RRM12 complex with RNA, only RRM1 is involved in RNA binding and recognizes a dinucleotide sequence.

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  3. Small-molecule exhibits anti-tumor activity by targeting the RNA m6A reader IGF2BP3 in ovarian cancer. Shu C, Gu MH, Zeng C, Shao WG, Li HY, Ma XH, Li MX, Cao YY, Zhang MJ, Zhao W, Zhao SL. Am J Cancer Res 13 4888-4902 (2023)


Reviews citing this publication (4)

  1. IGF2BP3 From Physiology to Cancer: Novel Discoveries, Unsolved Issues, and Future Perspectives. Mancarella C, Scotlandi K. Front Cell Dev Biol 7 363 (2019)
  2. The biological function of IGF2BPs and their role in tumorigenesis. Du QY, Zhu ZM, Pei DS. Invest New Drugs 39 1682-1693 (2021)
  3. IGF2BPs as novel m6A readers: Diverse roles in regulating cancer cell biological functions, hypoxia adaptation, metabolism, and immunosuppressive tumor microenvironment. Duan M, Liu H, Xu S, Yang Z, Zhang F, Wang G, Wang Y, Zhao S, Jiang X. Genes Dis 11 890-920 (2024)
  4. Role of IGF2BPs in head and neck squamous cell carcinoma. Wu K, Chang F, Li W, Su T, Lei D. Front Oncol 12 1003808 (2022)

Articles citing this publication (8)

  1. CircCDKN2B-AS1 interacts with IMP3 to stabilize hexokinase 2 mRNA and facilitate cervical squamous cell carcinoma aerobic glycolysis progression. Zhang Y, Zhao L, Yang S, Cen Y, Zhu T, Wang L, Xia L, Liu Y, Zou J, Xu J, Li Y, Cheng X, Lu W, Wang X, Xie X. J Exp Clin Cancer Res 39 281 (2020)
  2. Combinatorial recognition of clustered RNA elements by the multidomain RNA-binding protein IMP3. Schneider T, Hung LH, Aziz M, Wilmen A, Thaum S, Wagner J, Janowski R, Müller S, Schreiner S, Friedhoff P, Hüttelmaier S, Niessing D, Sattler M, Schlundt A, Bindereif A. Nat Commun 10 2266 (2019)
  3. LncRNA-PACERR induces pro-tumour macrophages via interacting with miR-671-3p and m6A-reader IGF2BP2 in pancreatic ductal adenocarcinoma. Liu Y, Shi M, He X, Cao Y, Liu P, Li F, Zou S, Wen C, Zhan Q, Xu Z, Wang J, Sun B, Shen B. J Hematol Oncol 15 52 (2022)
  4. RNA-Binding Proteins at the Host-Pathogen Interface Targeting Viral Regulatory Elements. Embarc-Buh A, Francisco-Velilla R, Martinez-Salas E. Viruses 13 952 (2021)
  5. Sestrin2-mediated disassembly of stress granules dampens aerobic glycolysis to overcome glucose starvation. Li M, Thorne RF, Wang R, Cao L, Cheng F, Sun X, Wu M, Ma J, Liu L. Cell Death Discov 9 127 (2023)
  6. Association of circulating tumor cells and IMP3 expression with metastasis of osteosarcoma. Dai S, Shao X, Wei Q, Du S, Hou C, Li H, Jin D. Front Oncol 13 819357 (2023)
  7. IGF2BP3 as a Prognostic Biomarker in Well-Differentiated/Dedifferentiated Liposarcoma. Klingbeil KD, Tang JP, Graham DS, Lofftus SY, Jaiswal AK, Lin TL, Frias C, Chen LY, Nakasaki M, Dry SM, Crompton JG, Eilber FC, Rao DS, Kalbasi A, Kadera BE. Cancers (Basel) 15 4489 (2023)
  8. Insight into the Structural Basis for Dual Nucleic Acid-Recognition by the Scaffold Attachment Factor B2 Protein. Korn SM, Von Ehr J, Dhamotharan K, Tants JN, Abele R, Schlundt A. Int J Mol Sci 24 3286 (2023)


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