› 6gx7

X-ray diffraction
3.19Å resolution

Tubulin-CopN-alphaRep complex

Released:
DOI: 10.2210/pdb6gx7/pdb
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PDB entry 6gx7 coloured by chain, front view.
PDB entry 6gx7 coloured by chain, side view.
PDB entry 6gx7 coloured by chain, top view.
Source organisms:
Primary publication:
Insight into microtubule nucleation from tubulin-capping proteins.
Campanacci V, Urvoas A, Cantos-Fernandes S, Aumont-Nicaise M, Arteni AA, Velours C, Valerio-Lepiniec M, Dreier B, Plückthun A, Pilon A, Poüs C, Minard P, Gigant B
Proc Natl Acad Sci U S A 116 9859-9864 (2019)
PMID: 31036638

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-112165 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
iiiA5 ALPHAREP Chains: E, F
Low calcium response E Chains: G, H

Ligands and Environments

6 bound ligands:
Ligand GTP 4 x GTP
Ligand MG 4 x MG
Ligand SO4 2 x SO4
Ligand MES 4 x MES
Ligand GOL 3 x GOL
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 148.06Å b: 95.85Å c: 158.06Å
α: 90° β: 101.49° γ: 90°
R-values:
R R work R free
0.175 0.173 0.21
Expression system: Escherichia coli

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Citations

2 review citations

The Mechanism of Tubulin Assembly into Microtubules: Insights from Structural Studies.
Knossow et al. (2020)
1 more

3 mentions without citation

Comprehensive Analysis of Binding Sites in Tubulin.
Mühlethaler et al. (2021)
2 more

PDB-REDO

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