6gz3

Electron Microscopy
3.6Å resolution

tRNA translocation by the eukaryotic 80S ribosome and the impact of GTP hydrolysis, Translocation-intermediate-POST-1 (TI-POST-1)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 86-mer (preferred)
PDBe Complex ID:
PDB-CPX-110575 (preferred)
Entry contents:
79 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (86 distinct):
ribosomal protein uS3 Chain: BD
Molecule details ›
Chain: BD
Length: 220 amino acids
Theoretical weight: 24.36 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein uS7 Chain: BF
Molecule details ›
Chain: BF
Length: 190 amino acids
Theoretical weight: 21.42 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TFM5 (Residues: 15-204; Coverage: 93%)
Gene name: RPS5
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein eS10 Chain: BK
Small ribosomal subunit protein eS12 Chain: BM
ribosomal protein uS19 Chain: BP
ribosomal protein uS9 Chain: BQ
Small ribosomal subunit protein eS17 Chain: BR
Small ribosomal subunit protein uS13 Chain: BS
ribosomal protein eS19 Chain: BT
ribosomal protein uS10 Chain: BU
Small ribosomal subunit protein eS25 Chain: BZ
Small ribosomal subunit protein eS28 Chain: Bc
Small ribosomal subunit protein uS14 Chain: Bd
Small ribosomal subunit protein eS31 Chain: Bf
Small ribosomal subunit protein RACK1 Chain: Bg
ribosomal protein uS2 Chain: BA
Small ribosomal subunit protein eS1 Chain: BB
ribosomal protein uS5 Chain: BC
ribosomal protein eS4 Chain: BE
Small ribosomal subunit protein eS6 Chain: BG
Small ribosomal subunit protein eS7 Chain: BH
ribosomal protein eS8 Chain: BI
Small ribosomal subunit protein uS4 Chain: BJ
Small ribosomal subunit protein uS17 Chain: BL
Small ribosomal subunit protein uS15 Chain: BN
ribosomal protein uS11 Chain: BO
ribosomal protein eS21 Chain: BV
Small ribosomal subunit protein uS8 Chain: BW
Small ribosomal subunit protein uS12 Chain: BX
ribosomal protein eS24 Chain: BY
ribosomal protein eS26 Chain: Ba
ribosomal protein eS27 Chain: Bb
ribosomal protein eS30 Chain: Be
Large ribosomal subunit protein uL2 Chain: AA
ribosomal protein uL3 Chain: AB
ribosomal protein uL4 Chain: AC
ribosomal protein uL18 Chain: AD
ribosomal protein eL6 Chain: AE
ribosomal protein uL30 Chain: AF
ribosomal protein eL8 Chain: AG
ribosomal protein uL6 Chain: AH
Large ribosomal subunit protein uL16 Chain: AI
Large ribosomal subunit protein uL5 Chain: AJ
ribosomal protein eL13 Chain: AL
ribosomal protein eL14 Chain: AM
Large ribosomal subunit protein eL15 Chain: AN
ribosomal protein uL13 Chain: AO
ribosomal protein uL22 Chain: AP
ribosomal protein eL18 Chain: AQ
ribosomal protein eL19 Chain: AR
ribosomal protein eL20 Chain: AS
Large ribosomal subunit protein eL21 Chain: AT
ribosomal protein eL22 Chain: AU
Large ribosomal subunit protein uL14 Chain: AV
Large ribosomal subunit protein eL24 Chain: AW
ribosomal protein uL23 Chain: AX
Large ribosomal subunit protein uL24 Chain: AY
Large ribosomal subunit protein eL27 Chain: AZ
ribosomal protein uL15 Chain: Aa
ribosomal protein eL29 Chain: Ab
ribosomal protein eL30 Chain: Ac
Large ribosomal subunit protein eL31 Chain: Ad
Large ribosomal subunit protein eL32 Chain: Ae
ribosomal protein eL33 Chain: Af
ribosomal protein eL34 Chain: Ag
ribosomal protein uL29 Chain: Ah
ribosomal protein eL36 Chain: Ai
Large ribosomal subunit protein eL37 Chain: Aj
ribosomal protein eL38 Chain: Ak
Large ribosomal subunit protein eL39 Chain: Al
ribosomal protein eL40 Chain: Am
ribosomal protein eL41 Chain: An
Large ribosomal subunit protein eL42 Chain: Ao
Large ribosomal subunit protein eL43 Chain: Ap
ribosomal protein eL28 Chain: At
Large ribosomal subunit protein uL1 Chain: Au
Large ribosomal subunit protein uL11 Chain: Aq
ribosomal protein uL10 Chain: AK
eukaryotic elongation factor 2 (eEF2) Chain: Ct
28S ribosomal RNA Chain: A2
ap/P-site tRNA Chain: Bv
Messenger RNA Chain: Bx
pe/E-site-tRNA Chain: Bw
18S Ribosomal RNA Chain: B1
5.8S ribosomal RNA Chain: A3
5S ribosomal RNA Chain: A4

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-0098