› 6h16

X-ray diffraction
2.9Å resolution

Structure of LRP6 P3E3P4E4 in complex with VHH L-P2-D07

Released:
DOI: 10.2210/pdb6h16/pdb
PDB entry 6h16 coloured by chain, front view.
PDB entry 6h16 coloured by chain, side view.
PDB entry 6h16 coloured by chain, top view.
Source organisms:
Primary publication:
Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells.
Fenderico N, van Scherpenzeel RC, Goldflam M, Proverbio D, Jordens I, Kralj T, Stryeck S, Bass TZ, Hermans G, Ullman C, Aastrup T, Gros P, Maurice MM
OpenAccess logo Nat Commun 10 365 (2019)
PMID: 30664649

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208150 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Low-density lipoprotein receptor-related protein 6 Chain: A
Molecule details ›
Chain: A
Length: 618 amino acids
Theoretical weight: 69.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75581 (Residues: 630-1244; Coverage: 39%)
Gene name: LRP6
Sequence domains:
Structure domains: TolB, C-terminal domain
VHH L-P2-D07 Chain: B
Molecule details ›
Chain: B
Length: 119 amino acids
Theoretical weight: 13.09 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 92.795Å b: 105.954Å c: 164.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.252
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

13 review citations

Mutations and mechanisms of WNT pathway tumour suppressors in cancer.
Bugter et al. (2021)
12 more