› 6h5n

X-ray diffraction
3.23Å resolution

Plasmodium falciparum Pfs48/45 C-terminal domain bound to monoclonal antibody 85RF45.1

Released:
DOI: 10.2210/pdb6h5n/pdb
PDB entry 6h5n coloured by chain, front view.
PDB entry 6h5n coloured by chain, side view.
PDB entry 6h5n coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody.
Lennartz F, Brod F, Dabbs R, Miura K, Mekhaiel D, Marini A, Jore MM, Søgaard MM, Jørgensen T, de Jongh WA, Sauerwein RW, Long CA, Biswas S, Higgins MK
OpenAccess logo Nat Commun 9 3822 (2018)
PMID: 30237518

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212584 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Gametocyte surface protein P45/48 Chains: A, D
Molecule details ›
Chains: A, D
Length: 136 amino acids
Theoretical weight: 15.07 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q8I6T1 (Residues: 293-428; Coverage: 32%)
Gene names: PF13_0247, PF3D7_1346700, PF45/48, PFS45-48, PFS45/48
Sequence domains: Sexual stage antigen s48/45 domain
Antibody 85RF45.1 light chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 212 amino acids
Theoretical weight: 23.21 KDa
Source organism: Rattus norvegicus
Expression system: Rattus norvegicus
Structure domains: Immunoglobulins
Antibody 85RF45.1 heavy chain Chains: C, F
Molecule details ›
Chains: C, F
Length: 220 amino acids
Theoretical weight: 23.86 KDa
Source organism: Rattus norvegicus
Expression system: Rattus norvegicus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Unit cell:
a: 59.69Å b: 165.39Å c: 189.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.261 0.282
Expression systems:
  • Drosophila melanogaster
  • Rattus norvegicus

Quick links

Citations

6 review citations

Immunity against sexual stage Plasmodium falciparum and Plasmodium vivax parasites.
de Jong et al. (2020)
5 more

5 mentions without citation

Structural vaccinology of malaria transmission-blocking vaccines.
Patel et al. (2021)
4 more