› 6h6s

X-ray diffraction
1.45Å resolution

Sad phasing on nickel-substituted human carbonic anhydrase II

Released:
DOI: 10.2210/pdb6h6s/pdb
PDB entry 6h6s coloured by chain, front view.
PDB entry 6h6s coloured by chain, side view.
PDB entry 6h6s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Non-crystallographic symmetry in proteins: Jahn-Teller-like and Butterfly-like effects?
Silva JM, Giuntini S, Cerofolini L, Geraldes CFGC, Macedo AL, Ravera E, Fragai M, Luchinat C, Calderone V
J Biol Inorg Chem 24 91-101 (2019)
PMID: 30470900

Function and Biology Details

Reactions catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Urea = cyanamide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133681 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 29.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00918 (Residues: 2-260; Coverage: 100%)
Gene name: CA2
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 73.48Å b: 41.18Å c: 84.04Å
α: 90° β: 109.25° γ: 90°
R-values:
R R work R free
0.152 0.15 0.204
Expression system: Escherichia coli

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Citations

1 mention without citation

Recent High-Resolution Structures of Amyloids Involved in Neurodegenerative Diseases.
Diaz-Espinoza R. (2021)