› 6h8h

X-ray diffraction
1.9Å resolution

Molybdenum storage protein - in a recombinant and in vivo-like form

Released:
DOI: 10.2210/pdb6h8h/pdb
PDB entry 6h8h coloured by chain, front view.
PDB entry 6h8h coloured by chain, side view.
PDB entry 6h8h coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
The molybdenum storage protein - A bionanolab for creating experimentally alterable polyoxomolybdate clusters.
Brünle S, Poppe J, Hail R, Demmer U, Ermler U
J Inorg Biochem 189 172-179 (2018)
PMID: 30278367

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-551327 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Molybdenum storage protein subunit beta Chain: B
Molecule details ›
Chain: B
Length: 269 amino acids
Theoretical weight: 28.25 KDa
Source organism: Azotobacter vinelandii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84253 (Residues: 2-270; Coverage: 100%)
Gene names: Avin_43210, mosB
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like
Molybdenum storage protein subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 29.25 KDa
Source organism: Azotobacter vinelandii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84308 (Residues: 2-276; Coverage: 100%)
Gene names: Avin_43200, mosA
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

8 bound ligands:
Ligand 8M0 2 x 8M0
Ligand FUQ 1 x FUQ
Ligand GX2 1 x GX2
Ligand PO4 2 x PO4
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand MOO 1 x MOO
Ligand M27 1 x M27
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6322
Unit cell:
a: 117.1Å b: 117.1Å c: 235.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.206 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Interweaving Disciplines to Advance Chemistry: Applying Polyoxometalates in Biology.
Gumerova et al. (2021)
2 other articles