› 6ha7

X-ray diffraction
2.49Å resolution

Crystal structure of the BiP NBD and MANF complex

Released:
DOI: 10.2210/pdb6ha7/pdb
PDB entry 6ha7 coloured by chain, front view.
PDB entry 6ha7 coloured by chain, side view.
PDB entry 6ha7 coloured by chain, top view.
Source organisms:
Primary publication:
MANF antagonizes nucleotide exchange by the endoplasmic reticulum chaperone BiP.
Yan Y, Rato C, Rohland L, Preissler S, Ron D
OpenAccess logo Nat Commun 10 541 (2019)
PMID: 30710085

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124345 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endoplasmic reticulum chaperone BiP Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 42.64 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: G3I8R9 (Residues: 28-413; Coverage: 61%)
Gene names: GRP78, HSPA5, I79_019946
Sequence domains: Hsp70 protein
Mesencephalic astrocyte-derived neurotrophic factor Chains: C, D
Molecule details ›
Chains: C, D
Length: 158 amino acids
Theoretical weight: 18.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9CXI5 (Residues: 22-179; Coverage: 100%)
Gene names: Armet, Manf
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 58.42Å b: 60.96Å c: 96.11Å
α: 81.13° β: 88.33° γ: 74.33°
R-values:
R R work R free
0.228 0.226 0.256
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Protective Functions of Reactive Astrocytes Following Central Nervous System Insult.
Linnerbauer et al. (2020)
13 more