› 6he8

Electron Microscopy
6.86Å resolution

PAN-proteasome in state 1

Released:
DOI: 10.2210/pdb6he8/pdb
PDB entry 6he8 coloured by chain, front view.
PDB entry 6he8 coloured by chain, side view.
PDB entry 6he8 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle.
Majumder P, Rudack T, Beck F, Danev R, Pfeifer G, Nagy I, Baumeister W
OpenAccess logo Proc Natl Acad Sci U S A 116 534-539 (2019)
PMID: 30559193
Related structures: EMD-0212

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 34-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128008 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit alpha Chains: A, B, C, D, E, F, G, a, b, c, d, e, f, g
Molecule details ›
Chains: A, B, C, D, E, F, G, a, b, c, d, e, f, g
Length: 242 amino acids
Theoretical weight: 27.16 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29760 (Residues: 5-246; Coverage: 98%)
Gene names: AF_0490, psmA
Sequence domains:
Proteasome subunit beta Chains: 1, 2, 3, 4, 5, 6, 7, h, i, j, k, l, m, n
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, h, i, j, k, l, m, n
Length: 202 amino acids
Theoretical weight: 22.13 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9P996 (Residues: 12-213; Coverage: 95%)
Gene names: AF_0481, psmB
Sequence domains: Proteasome subunit
Proteasome-activating nucleotidase Chains: H, I, J, K, L, M
Molecule details ›
Chains: H, I, J, K, L, M
Length: 390 amino acids
Theoretical weight: 44.1 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28303 (Residues: 9-398; Coverage: 98%)
Gene names: AF_1976, pan
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 5 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.86Å
Relevant EMDB volumes: EMD-0212
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The molecular principles governing the activity and functional diversity of AAA+ proteins.
Puchades et al. (2020)
11 more

1 mention without citation

Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle.
Majumder et al. (2019)