› 6hjk

X-ray diffraction
2.4Å resolution

Crystal Structure of Aurora-A L210C catalytic domain in complex with ASDO2

Released:
DOI: 10.2210/pdb6hjk/pdb
PDB entry 6hjk coloured by chain, front view.
PDB entry 6hjk coloured by chain, side view.
PDB entry 6hjk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Type II Kinase Inhibitors Targeting Cys-Gatekeeper Kinases Display Orthogonality with Wild Type and Ala/Gly-Gatekeeper Kinases.
Ocasio CA, Warkentin AA, McIntyre PJ, Barkovich KJ, Vesely C, Spencer J, Shokat KM, Bayliss R
ACS Chem Biol 13 2956-2965 (2018)
PMID: 30239186

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aurora kinase A Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 32.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand G7W 1 x G7W
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3221
Unit cell:
a: 88.769Å b: 88.769Å c: 77.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.217 0.252
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Phosphoproteomics Meets Chemical Genetics: Approaches for Global Mapping and Deciphering the Phosphoproteome.
Jurcik et al. (2020)
1 more

2 mentions without citation

Structural features of the protein kinase domain and targeted binding by small-molecule inhibitors.
Arter et al. (2022)
1 more