› 6hpn

X-ray diffraction
2.6Å resolution

Crystal structure of Borrelia spielmanii WP_012665240 (BSA64) - an orthologous protein to B. burgdorferi BBA64

Released:
DOI: 10.2210/pdb6hpn/pdb
PDB entry 6hpn coloured by chain, front view.
PDB entry 6hpn coloured by chain, side view.
PDB entry 6hpn coloured by chain, top view.
Source organism: Borreliella spielmanii A14S
Primary publication:
Structural analysis of the outer surface proteins from Borrelia burgdorferi paralogous gene family 54 that are thought to be the key players in the pathogenesis of Lyme disease.
Brangulis K, Akopjana I, Petrovskis I, Kazaks A, Tars K
J Struct Biol 210 107490 (2020)
PMID: 32135236

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antigen, P35 Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.88 KDa
Source organism: Borreliella spielmanii A14S
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C0RC68 (Residues: 102-322; Coverage: 69%)
Gene name: BSPA14S_A0059
Sequence domains: Borrelia Bbcrasp-1 domain containing protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 77.442Å b: 77.442Å c: 104.136Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Host tropism determination by convergent evolution of immunological evasion in the Lyme disease system.
Hart et al. (2021)
3 more

1 mention without citation

The surface lipoproteins of gram-negative bacteria: Protectors and foragers in harsh environments.
Cole et al. (2021)