6hr1

X-ray diffraction
1.9Å resolution

Crystal structure of the YFPnano fusion protein

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-139498 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Green fluorescent protein; Myosin light chain kinase 2, skeletal/cardiac muscle; Unconventional myosin-X Chains: A, B
Molecule details ›
Chains: A, B
Length: 434 amino acids
Theoretical weight: 49.2 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P79114 (Residues: 806-806, 826-837; Coverage: 1%)
  • Canonical: P07313 (Residues: 581-599; Coverage: 3%)
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1, GFP, MYLK2, MYO10
Sequence domains:

Ligands and Environments

1 modified residue:

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 53.7Å b: 117.8Å c: 84.5Å
α: 90° β: 99.9° γ: 90°
R-values:
R R work R free
0.182 0.181 0.214
Expression system: Escherichia coli BL21(DE3)