› 6hr4

X-ray diffraction
1.19Å resolution

Apo form of penicillin-binding protein 3 from P. aeruginosa

Released:
DOI: 10.2210/pdb6hr4/pdb
PDB entry 6hr4 coloured by chain, front view.
PDB entry 6hr4 coloured by chain, side view.
PDB entry 6hr4 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Bellini D, Dowson CG

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan D,D-transpeptidase FtsI Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 57.87 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3XD46 (Residues: 50-579; Coverage: 92%)
Gene names: PA4418, ftsI, pbpB
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 67.548Å b: 82.14Å c: 88.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.226
Expression system: Escherichia coli BL21(DE3)

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