› 6hr5

X-ray diffraction
2.91Å resolution

Structure of the S1_25 family sulfatase module of the rhamnosidase FA22250 from Formosa agariphila

Released:
DOI: 10.2210/pdb6hr5/pdb
PDB entry 6hr5 coloured by chain, front view.
PDB entry 6hr5 coloured by chain, side view.
PDB entry 6hr5 coloured by chain, top view.
Source organism: Formosa agariphila KMM 3901
Primary publication:
A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan.
Reisky L, Préchoux A, Zühlke MK, Bäumgen M, Robb CS, Gerlach N, Roret T, Stanetty C, Larocque R, Michel G, Song T, Markert S, Unfried F, Mihovilovic MD, Trautwein-Schult A, Becher D, Schweder T, Bornscheuer UT, Hehemann JH
Nat Chem Biol 15 803-812 (2019)
PMID: 31285597

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional sulfatase/alpha-L-rhamnosidase Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 54.34 KDa
Source organism: Formosa agariphila KMM 3901
Expression system: Escherichia coli
UniProt:
  • Canonical: T2KM26 (Residues: 22-466; Coverage: 39%)
Gene name: BN863_22250
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 52.879Å b: 48.871Å c: 92.651Å
α: 90° β: 105.64° γ: 90°
R-values:
R R work R free
0.324 0.322 0.361
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Marine Polysaccharides: Occurrence, Enzymatic Degradation and Utilization.
Bäumgen et al. (2021)
6 more

1 mention without citation

Sulfatases: Critical Enzymes for Algal Polysaccharide Processing.
Hettle et al. (2022)