› 6hz4

Electron Microscopy
3.6Å resolution

Structure of McrBC without DNA binding domains (one half of the full complex)

Released:
DOI: 10.2210/pdb6hz4/pdb
PDB entry 6hz4 coloured by chain, front view.
PDB entry 6hz4 coloured by chain, side view.
PDB entry 6hz4 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure-based mechanism for activation of the AAA+ GTPase McrB by the endonuclease McrC.
Nirwan N, Itoh Y, Singh P, Bandyopadhyay S, Vinothkumar KR, Amunts A, Saikrishnan K
OpenAccess logo Nat Commun 10 3058 (2019)
PMID: 31296862
Related structures: EMD-0310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147169 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type IV methyl-directed restriction enzyme EcoKMcrB subunit Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 307 amino acids
Theoretical weight: 35.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P15005 (Residues: 162-459; Coverage: 65%)
Gene names: JW5871, b4346, mcrB, rglB
Sequence domains: AAA domain (dynein-related subfamily)
Type IV methyl-directed restriction enzyme EcoKMcrBC Chains: M, N
Molecule details ›
Chains: M, N
Length: 348 amino acids
Theoretical weight: 40.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P15006 (Residues: 1-348; Coverage: 100%)
Gene names: JW5789, b4345, mcrC
Sequence domains: McrBC 5-methylcytosine restriction system component

Ligands and Environments

3 bound ligands:
Ligand GNP 3 x GNP
Ligand MG 3 x MG
Ligand GDP 3 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-0310
Expression system: Escherichia coli

Quick links

Citations

1 review citation

AAA+ ATPases: structural insertions under the magnifying glass.
Jessop et al. (2021)
8 other articles

1 mention without citation

Insights into the structure-function relationship of the NorQ/NorD chaperones from Paracoccus denitrificans reveal shared principles of interacting MoxR AAA+/VWA domain proteins.
Kahle et al. (2023)