› 6i3a

X-ray diffraction
1.45Å resolution

Crystal structure of v22Pizza6-AYW, a circularly permuted designer protein

Released:
DOI: 10.2210/pdb6i3a/pdb
PDB entry 6i3a coloured by chain, front view.
PDB entry 6i3a coloured by chain, side view.
PDB entry 6i3a coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Influence of circular permutations on the structure and stability of a six-fold circular symmetric designer protein.
Mylemans B, Noguchi H, Deridder E, Lescrinier E, Tame JRH, Voet ARD
Protein Sci 29 2375-2386 (2020)
PMID: 33006397

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
v22Pizza6-AYW Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 25.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21

Ligands and Environments

1 bound ligand:
Ligand BR 7 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 49.196Å b: 56.892Å c: 69.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.209
Expression system: Escherichia coli BL21

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Citations

1 review citation

Glycoside hydrolases in the biodegradation of lignocellulosic biomass.
Lu et al. (2023)
3 other articles

1 mention without citation

Influence of circular permutations on the structure and stability of a six-fold circular symmetric designer protein.
Mylemans et al. (2020)