› 6i5z

X-ray diffraction
3Å resolution

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + (S)-scoulerine = S-adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
S-adenosyl-L-methionine + 5,8,13,13a-tetrahydrocolumbamine = S-adenosyl-L-homocysteine + tetrahydropalmatine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Scoulerine-9-O-methyltransferase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 393 amino acids
Theoretical weight: 42.89 KDa
Source organism: Papaver somniferum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I3V6A7 (Residues: 1-390; Coverage: 100%)
Gene names: PSMT1, SOMT1
Sequence domains:
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH

Cofactor: Ligand SAM 1 x SAM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 111.148Å b: 111.148Å c: 302.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.203 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links