› 6i8s

X-ray diffraction
2.9Å resolution

Discovery and characterisation of an antibody that selectively modulates the inhibitory activity of plasminogen activator inhibitor-1

Released:
DOI: 10.2210/pdb6i8s/pdb
PDB entry 6i8s coloured by chain, front view.
PDB entry 6i8s coloured by chain, side view.
PDB entry 6i8s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery and characterisation of an antibody that selectively modulates the inhibitory activity of plasminogen activator inhibitor-1.
Vousden KA, Lundqvist T, Popovic B, Naiman B, Carruthers AM, Newton P, Johnson DJD, Pomowski A, Wilkinson T, Dufner P, de Mendez I, Mallinder PR, Murray C, Strain M, Connor J, Murray LA, Sleeman MA, Lowe DC, Huntington JA, Vaughan TJ
OpenAccess logo Sci Rep 9 1605 (2019)
PMID: 30733557

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209237 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Plasminogen activator inhibitor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 379 amino acids
Theoretical weight: 42.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
HEAVY CHAIN OF FAB FRAGMENT FROM AN PAI-1 ANTIBODY Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 223 amino acids
Theoretical weight: 23.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
LIGHT CHAIN OF FAB FRAGMENT FROM AN PAI-1 ANTIBODY Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 214 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 90.22Å b: 89.66Å c: 249.77Å
α: 90° β: 99.35° γ: 90°
R-values:
R R work R free
0.22 0.219 0.243
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The Role of Metabolic Syndrome in Endometrial Cancer: A Review.
Yang et al. (2019)
7 more

3 mentions without citation

Targeting PAI-1 in Cardiovascular Disease: Structural Insights Into PAI-1 Functionality and Inhibition.
Sillen et al. (2020)
2 more