› 6i9t

X-ray diffraction
1.2Å resolution

Two minutes iron loaded Rana Catesbeiana H' ferritin variant H54N

Released:
DOI: 10.2210/pdb6i9t/pdb
PDB entry 6i9t coloured by chain, front view.
PDB entry 6i9t coloured by chain, side view.
PDB entry 6i9t coloured by chain, top view.
Source organism: Lithobates catesbeianus
Primary publication:
Effect of the point mutation H54N on the ferroxidase process of Rana catesbeiana H' ferritin.
Pozzi C, Di Pisa F, Lalli D, Rosa C, Turano P, Mangani S
J Inorg Biochem 197 110697 (2019)
PMID: 31075719

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-530297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin, middle subunit Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.6 KDa
Source organism: Lithobates catesbeianus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07798 (Residues: 1-176; Coverage: 100%)
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand FE2 1 x FE2
Ligand MG 12 x MG
Ligand CL 9 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14, ELETTRA BEAMLINE 5.2R
Spacegroup: F432
Unit cell:
a: 183.83Å b: 183.83Å c: 183.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.137
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural Insights Into the Effects of Interactions With Iron and Copper Ions on Ferritin From the Blood Clam Tegillarca granosa.
Ming et al. (2022)