6ia6 Citations

The Elongator subunit Elp3 is a non-canonical tRNA acetyltransferase.

Abstract

The Elongator complex catalyzes posttranscriptional tRNA modifications by attaching carboxy-methyl (cm5) moieties to uridine bases located in the wobble position. The catalytic subunit Elp3 is highly conserved and harbors two individual subdomains, a radical S-adenosyl methionine (rSAM) and a lysine acetyltransferase (KAT) domain. The details of its modification reaction cycle and particularly the substrate specificity of its KAT domain remain elusive. Here, we present the co-crystal structure of bacterial Elp3 (DmcElp3) bound to an acetyl-CoA analog and compare it to the structure of a monomeric archaeal Elp3 from Methanocaldococcus infernus (MinElp3). Furthermore, we identify crucial active site residues, confirm the importance of the extended N-terminus for substrate recognition and uncover the specific induction of acetyl-CoA hydrolysis by different tRNA species. In summary, our results establish the clinically relevant Elongator subunit as a non-canonical acetyltransferase and genuine tRNA modification enzyme.

Reviews - 6ia6 mentioned but not cited (1)

  1. How Elongator Acetylates tRNA Bases. Abbassi NE, Biela A, Glatt S, Lin TY. Int J Mol Sci 21 E8209 (2020)

Articles - 6ia6 mentioned but not cited (1)



Reviews citing this publication (7)

  1. Iron-sulfur clusters as inhibitors and catalysts of viral replication. Honarmand Ebrahimi K, Ciofi-Baffoni S, Hagedoorn PL, Nicolet Y, Le Brun NE, Hagen WR, Armstrong FA. Nat Chem 14 253-266 (2022)
  2. Iron in Translation: From the Beginning to the End. Romero AM, Martínez-Pastor MT, Puig S. Microorganisms 9 1058 (2021)
  3. Anticodon Wobble Uridine Modification by Elongator at the Crossroad of Cell Signaling, Differentiation, and Diseases. Hermand D. Epigenomes 4 7 (2020)
  4. Plant Elongator-Protein Complex of Diverse Activities Regulates Growth, Development, and Immune Responses. Jarosz M, Van Lijsebettens M, Woloszynska M. Int J Mol Sci 21 E6912 (2020)
  5. The diverse structural modes of tRNA binding and recognition. Biela A, Hammermeister A, Kaczmarczyk I, Walczak M, Koziej L, Lin TY, Glatt S. J Biol Chem 299 104966 (2023)
  6. Highlighting the Unique Roles of Radical S-Adenosylmethionine Enzymes in Methanogenic Archaea. Boswinkle K, McKinney J, Allen KD. J Bacteriol 204 e0019722 (2022)
  7. "Superwobbling" and tRNA-34 Wobble and tRNA-37 Anticodon Loop Modifications in Evolution and Devolution of the Genetic Code. Lei L, Burton ZF. Life (Basel) 12 252 (2022)

Articles citing this publication (26)