6ibw

X-ray diffraction
2.8Å resolution

Crh5 transglycosylase in complex with NAG

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-184984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Probable glycosidase crf1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 26.6 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8J0P4 (Residues: 22-266; Coverage: 65%)
Gene names: AFUA_1G16190, crf1
Sequence domains: Glycosyl hydrolases family 16

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 55.39Å b: 75.3Å c: 117.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.24
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'