› 6icg

X-ray diffraction
1.15Å resolution

Grb2 SH2 domain in phosphopeptide free form

Released:
DOI: 10.2210/pdb6icg/pdb
PDB entry 6icg coloured by chain, front view.
PDB entry 6icg coloured by chain, side view.
PDB entry 6icg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional properties of Grb2 SH2 dimer in CD28 binding.
Hosoe Y, Numoto N, Inaba S, Ogawa S, Morii H, Abe R, Ito N, Oda M
OpenAccess logo Biophys Physicobiol 16 80-88 (2019)
PMID: 30923665

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Growth factor receptor-bound protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62993 (Residues: 60-152; Coverage: 43%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 79.55Å b: 79.55Å c: 69.635Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Recent Advances in Allogeneic CAR-T Cells.
Kim et al. (2020)
1 more

2 mentions without citation

Structural and functional properties of Grb2 SH2 dimer in CD28 binding.
Hosoe et al. (2019)
1 more