6idf

Electron Microscopy
2.7Å resolution

Cryo-EM structure of gamma secretase in complex with a Notch fragment

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis of Notch recognition by human γ-secretase.
Nature 565 192-197 (2019)
PMID: 30598546
Related structures: EMD-9648

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-155481 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
Nicastrin Chain: A
Molecule details ›
Chain: A
Length: 709 amino acids
Theoretical weight: 78.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 1-709; Coverage: 100%)
Gene names: KIAA0253, NCSTN, UNQ1874/PRO4317
Sequence domains:
Presenilin-1 Chain: B
Molecule details ›
Chain: B
Length: 467 amino acids
Theoretical weight: 52.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 1-467; Coverage: 100%)
Gene names: AD3, PS1, PSEN1, PSNL1
Sequence domains: Presenilin
Gamma-secretase subunit APH-1A Chain: C
Gamma-secretase subunit PEN-2 Chain: D
Notch 1 intracellular domain Chain: E

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-9648
Expression system: Homo sapiens