› 6iej

X-ray diffraction
2.21Å resolution

The C2 domain of cytosolic phospholipase A2 alpha bound to phosphatidylcholine

Released:
DOI: 10.2210/pdb6iej/pdb
PDB entry 6iej coloured by chain, front view.
PDB entry 6iej coloured by chain, side view.
PDB entry 6iej coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase A2α.
Hirano Y, Gao YG, Stephenson DJ, Vu NT, Malinina L, Simanshu DK, Chalfant CE, Patel DJ, Brown RE
OpenAccess logo Elife 8 (2019)
PMID: 31050338

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic phospholipase A2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 127 amino acids
Theoretical weight: 14.31 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P49147 (Residues: 16-140; Coverage: 17%)
Gene names: CPLA2, PLA2G4, PLA2G4A
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 9 x CA
Ligand MG 2 x MG
Ligand HXG 3 x HXG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C222
Unit cell:
a: 108.294Å b: 187.388Å c: 68.754Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.249
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Roles of polyunsaturated fatty acids, from mediators to membranes.
Harayama et al. (2020)
1 more

1 mention without citation

Structural basis of phosphatidylcholine recognition by the C2-domain of cytosolic phospholipase A2α.
Hirano et al. (2019)