› 6ig5

X-ray diffraction
2.08Å resolution

Crystal structure of argininosuccinate lyase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb6ig5/pdb
PDB entry 6ig5 coloured by chain, front view.
PDB entry 6ig5 coloured by chain, side view.
PDB entry 6ig5 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure and biochemical study on argininosuccinate lyase from Mycobacterium tuberculosis.
Chen X, Chen J, Zhang W, Wang H, Liu X, Zhou W, Yang H, Rao Z
Biochem Biophys Res Commun 510 116-121 (2019)
PMID: 30665717

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 470 amino acids
Theoretical weight: 49.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPY7 (Residues: 1-470; Coverage: 100%)
Gene names: MTCY06H11.24, Rv1659, argH
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 115.349Å b: 120.352Å c: 155.422Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.2
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Arginine inhibition of the argininosuccinate lyases is conserved among three orders in cyanobacteria.
Katayama et al. (2022)