› 6ih7

X-ray diffraction
2.25Å resolution

Crystal structure of a standalone versatile EAL protein from Vibrio cholerae O395 - 3',3'-cGAMP bound form

Released:
DOI: 10.2210/pdb6ih7/pdb
PDB entry 6ih7 coloured by chain, front view.
PDB entry 6ih7 coloured by chain, side view.
PDB entry 6ih7 coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
Structures of c-di-GMP/cGAMP degrading phosphodiesterase VcEAL: identification of a novel conformational switch and its implication.
Yadav M, Pal K, Sen U
Biochem J 476 3333-3353 (2019)
PMID: 31647518

Function and Biology Details

Reaction catalysed: 
Cyclic di-3',5'-guanylate + H(2)O = 5'-phosphoguanylyl(3'->5')guanosine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-493844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EAL domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.03 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3AJ04 (Residues: 1-257; Coverage: 100%)
Gene name: VC0395_A1247
Sequence domains: EAL domain

Ligands and Environments

2 bound ligands:
Ligand 4BW 2 x 4BW
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER IMUS MICROFOCUS
Spacegroup: C2
Unit cell:
a: 157.899Å b: 42.6Å c: 73.33Å
α: 90° β: 94.91° γ: 90°
R-values:
R R work R free
0.184 0.181 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The ever-expanding world of bacterial cyclic oligonucleotide second messengers.
Yoon et al. (2021)
2 more