6ip1

Electron Microscopy
3.9Å resolution

alpha-SNAP-SNARE subcomplex in the whole 20S complex

Released:
Source organisms:
Primary publication:
Mechanistic insights into the SNARE complex disassembly.
OpenAccess logo Sci Adv 5 eaau8164 (2019)
PMID: 30989110
Related structures: EMD-9697

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-152475 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 1-94; Coverage: 81%)
Gene names: Syb2, Vamp2
Sequence domains: Synaptobrevin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 254 amino acids
Theoretical weight: 29.36 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 2-253; Coverage: 88%)
Gene names: Sap, Stx1a
Sequence domains:
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 102 amino acids
Theoretical weight: 11.57 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 1-100; Coverage: 49%)
Gene names: Snap, Snap25
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 83 amino acids
Theoretical weight: 9.28 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 126-206; Coverage: 39%)
Gene names: Snap, Snap25
Sequence domains: SNAP-25 family
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Alpha-soluble NSF attachment protein Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 309 amino acids
Theoretical weight: 34.8 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P81125 (Residues: 1-295; Coverage: 100%)
Gene names: NAPA, SNAPA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-9697
Expression system: Escherichia coli