6ip5

Electron Microscopy
3.9Å resolution

Cryo-EM structure of the CMV-stalled human 80S ribosome (Structure ii)

Released:

Function and Biology Details

Reaction catalysed:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 84-mer (preferred)
PDBe Complex ID:
PDB-CPX-140321 (preferred)
Entry contents:
77 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (83 distinct):
Large ribosomal subunit protein uL2 Chain: 1D
Molecule details ›
Chain: 1D
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL3 Chain: 1E
Molecule details ›
Chain: 1E
Length: 403 amino acids
Theoretical weight: 46.21 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL4 Chain: 1F
Molecule details ›
Chain: 1F
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL18 Chain: 1G
Molecule details ›
Chain: 1G
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL6 Chain: 1H
Molecule details ›
Chain: 1H
Length: 288 amino acids
Theoretical weight: 32.81 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL30 Chain: 2A
Molecule details ›
Chain: 2A
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL8 Chain: 2B
Molecule details ›
Chain: 2B
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL6 Chain: 2C
Molecule details ›
Chain: 2C
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
Ribosomal protein uL16-like Chain: 2D
Molecule details ›
Chain: 2D
Length: 214 amino acids
Theoretical weight: 24.57 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL5 Chain: 2E
Molecule details ›
Chain: 2E
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL13 Chain: 2F
Molecule details ›
Chain: 2F
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL14 Chain: 2G
Molecule details ›
Chain: 2G
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL15 Chain: 2H
Molecule details ›
Chain: 2H
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL13 Chain: 2I
Molecule details ›
Chain: 2I
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL22 Chain: 2J
Molecule details ›
Chain: 2J
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL18 Chain: 2K
Molecule details ›
Chain: 2K
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL19 Chain: 2L
Molecule details ›
Chain: 2L
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL20 Chain: 2M
Molecule details ›
Chain: 2M
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL21 Chain: 2N
Molecule details ›
Chain: 2N
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL22 Chain: 2O
Molecule details ›
Chain: 2O
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL14 Chain: 2P
Molecule details ›
Chain: 2P
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL24 Chain: 2Q
Molecule details ›
Chain: 2Q
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL23 Chain: 2R
Molecule details ›
Chain: 2R
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL24 Chain: 2S
Molecule details ›
Chain: 2S
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL27 Chain: 2T
Molecule details ›
Chain: 2T
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL15 Chain: 2U
Molecule details ›
Chain: 2U
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL29 Chain: 2V
Molecule details ›
Chain: 2V
Length: 159 amino acids
Theoretical weight: 17.8 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL30 Chain: 2W
Molecule details ›
Chain: 2W
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL31 Chain: 2X
Molecule details ›
Chain: 2X
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL32 Chain: 2Y
Molecule details ›
Chain: 2Y
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL33 Chain: 2Z
Molecule details ›
Chain: 2Z
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL34 Chain: 2a
Molecule details ›
Chain: 2a
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL29 Chain: 2b
Molecule details ›
Chain: 2b
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL36 Chain: 2c
Molecule details ›
Chain: 2c
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL37 Chain: 2d
Molecule details ›
Chain: 2d
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL38 Chain: 2e
Molecule details ›
Chain: 2e
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL39 Chain: 2f
Molecule details ›
Chain: 2f
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
Ubiquitin-ribosomal protein eL40 fusion protein Chain: 2g
Molecule details ›
Chain: 2g
Length: 128 amino acids
Theoretical weight: 14.76 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS32 Chain: 2h
Molecule details ›
Chain: 2h
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL42 Chain: 2i
Molecule details ›
Chain: 2i
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL43 Chain: 2j
Molecule details ›
Chain: 2j
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein eL28 Chain: 2k
Molecule details ›
Chain: 2k
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
Large ribosomal subunit protein uL1 Chain: 2l
Molecule details ›
Chain: 2l
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS2 Chain: 2n
Molecule details ›
Chain: 2n
Length: 295 amino acids
Theoretical weight: 32.88 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS1 Chain: 2o
Molecule details ›
Chain: 2o
Length: 264 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS3 Chain: 2p
Molecule details ›
Chain: 2p
Length: 243 amino acids
Theoretical weight: 26.73 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS4, X isoform Chain: 2q
Molecule details ›
Chain: 2q
Length: 263 amino acids
Theoretical weight: 29.65 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS7 Chain: 2r
Molecule details ›
Chain: 2r
Length: 204 amino acids
Theoretical weight: 22.91 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS7 Chain: 2s
Molecule details ›
Chain: 2s
Length: 194 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS8 Chain: 2t
Molecule details ›
Chain: 2t
Length: 208 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS10 Chain: 2u
Molecule details ›
Chain: 2u
Length: 165 amino acids
Theoretical weight: 18.93 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS17 Chain: 2v
Molecule details ›
Chain: 2v
Length: 158 amino acids
Theoretical weight: 18.47 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS19 Chain: 2w
Molecule details ›
Chain: 2w
Length: 145 amino acids
Theoretical weight: 17.08 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS9 Chain: 2x
Molecule details ›
Chain: 2x
Length: 146 amino acids
Theoretical weight: 16.48 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS17 Chain: 2y
Molecule details ›
Chain: 2y
Length: 135 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS13 Chain: 2z
Molecule details ›
Chain: 2z
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS19 Chain: 20
Molecule details ›
Chain: 20
Length: 145 amino acids
Theoretical weight: 16.09 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS10 Chain: 21
Molecule details ›
Chain: 21
Length: 119 amino acids
Theoretical weight: 13.4 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS21 Chain: 3A
Molecule details ›
Chain: 3A
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS12 Chain: 3B
Molecule details ›
Chain: 3B
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS26 Chain: 3C
Molecule details ›
Chain: 3C
Length: 115 amino acids
Theoretical weight: 13.05 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS28 Chain: 3D
Molecule details ›
Chain: 3D
Length: 69 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS14 Chain: 3E
Molecule details ›
Chain: 3E
Length: 56 amino acids
Theoretical weight: 6.69 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein RACK1 Chain: 3F
Molecule details ›
Chain: 3F
Length: 317 amino acids
Theoretical weight: 35.12 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS5 Chain: 3G
Molecule details ›
Chain: 3G
Length: 293 amino acids
Theoretical weight: 31.38 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS6 Chain: 3H
Molecule details ›
Chain: 3H
Length: 249 amino acids
Theoretical weight: 28.75 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS4 Chain: 3I
Molecule details ›
Chain: 3I
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS12 Chain: 3J
Molecule details ›
Chain: 3J
Length: 132 amino acids
Theoretical weight: 14.55 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS15 Chain: 3K
Molecule details ›
Chain: 3K
Length: 151 amino acids
Theoretical weight: 17.26 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS11 Chain: 3L
Molecule details ›
Chain: 3L
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein uS8 Chain: 3M
Molecule details ›
Chain: 3M
Length: 130 amino acids
Theoretical weight: 14.87 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS24 Chain: 3N
Molecule details ›
Chain: 3N
Length: 133 amino acids
Theoretical weight: 15.46 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS25 Chain: 3O
Molecule details ›
Chain: 3O
Length: 125 amino acids
Theoretical weight: 13.78 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS27 Chain: 3P
Molecule details ›
Chain: 3P
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Homo sapiens
Small ribosomal subunit protein eS30 Chain: 3Q
Molecule details ›
Chain: 3Q
Length: 59 amino acids
Theoretical weight: 6.67 KDa
Source organism: Homo sapiens
Ubiquitin-ribosomal protein eS31 fusion protein Chain: 3R
Molecule details ›
Chain: 3R
Length: 156 amino acids
Theoretical weight: 18 KDa
Source organism: Homo sapiens
nascent peptide Chain: zx
Molecule details ›
Chain: zx
Length: 17 amino acids
Theoretical weight: 1.87 KDa
Source organism: Homo sapiens
28S ribosomal RNA Chain: 1A
Molecule details ›
Chain: 1A
Length: 5070 nucleotides
Theoretical weight: 1640.18 KDa
Source organism: Homo sapiens
Sequence domains: Eukaryotic large subunit ribosomal RNA
5S ribosomal RNA Chain: 1B
Molecule details ›
Chain: 1B
Length: 121 nucleotides
Theoretical weight: 39 KDa
Source organism: Homo sapiens
Sequence domains: 5S ribosomal RNA
5.8S ribosomal RNA Chain: 1C
Molecule details ›
Chain: 1C
Length: 157 nucleotides
Theoretical weight: 50.45 KDa
Source organism: Homo sapiens
Sequence domains: 5.8S ribosomal RNA
18S Ribosomal RNA Chain: 2m
Molecule details ›
Chain: 2m
Length: 1869 nucleotides
Theoretical weight: 602.75 KDa
Source organism: Homo sapiens
Sequence domains: Eukaryotic small subunit ribosomal RNA
Messenger RNA Chain: zv
Molecule details ›
Chain: zv
Length: 6 nucleotides
Theoretical weight: 1.83 KDa
Source organism: Homo sapiens
P-site tRNA Chains: zu, zy
Molecule details ›
Chains: zu, zy
Length: 75 nucleotides
Theoretical weight: 24.19 KDa
Source organism: Homo sapiens
Sequence domains: tRNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-9701