› 6iqd

X-ray diffraction
2.84Å resolution

Crystal structure of Alcohol dehydrogenase from Geobacillus stearothermophilus

Released:
DOI: 10.2210/pdb6iqd/pdb
PDB entry 6iqd coloured by chain, front view.
PDB entry 6iqd coloured by chain, side view.
PDB entry 6iqd coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase.
Guo X, Feng Y, Wang X, Liu Y, Liu W, Li Q, Wang J, Xue S, Zhao ZK
Bioorg Med Chem Lett 29 1446-1449 (2019)
PMID: 31006524

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-544991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 339 amino acids
Theoretical weight: 36.25 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42327 (Residues: 1-339; Coverage: 100%)
Gene name: adh
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 71.442Å b: 149.186Å c: 269.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.291
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Enzymatic properties of alcohol dehydrogenase PedE_M.s. derived from Methylopila sp. M107 and its broad metal selectivity.
Xiao et al. (2023)
1 more

2 mentions without citation

Advanced Insights into Catalytic and Structural Features of the Zinc-Dependent Alcohol Dehydrogenase from Thauera aromatica.
Stark et al. (2022)
1 more