› 6iqw

Electron Microscopy
3.35Å resolution

Cryo-EM structure of Csm effector complex

Released:
DOI: 10.2210/pdb6iqw/pdb
PDB entry 6iqw coloured by chain, front view.
PDB entry 6iqw coloured by chain, side view.
PDB entry 6iqw coloured by chain, top view.
Source organism: Thermococcus onnurineus NA1
Primary publication:
Cryo-EM structure of Type III-A CRISPR effector complex.
Huo Y, Li T, Wang N, Dong Q, Wang X, Jiang T
Cell Res 28 1195-1197 (2018)
PMID: 30459428
Related structures: EMD-9708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-110418 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) Chain: A
Molecule details ›
Chain: A
Length: 777 amino acids
Theoretical weight: 88.13 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWB8 (Residues: 1-777; Coverage: 100%)
Gene names: TON_0893, cas10, csm1
Sequence domains:
CRISPR system Cms protein Csm2 Chain: B
Molecule details ›
Chain: B
Length: 196 amino acids
Theoretical weight: 22.41 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWB9 (Residues: 2-186; Coverage: 100%)
Gene name: TON_0894
Sequence domains: Csm2 Type III-A
CRISPR system Cms endoribonuclease Csm3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 290 amino acids
Theoretical weight: 32.72 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC0 (Residues: 1-290; Coverage: 100%)
Gene name: TON_0895
Sequence domains: RAMP superfamily
CRISPR system Cms protein Csm4 Chain: E
Molecule details ›
Chain: E
Length: 289 amino acids
Theoretical weight: 32.35 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC1 (Residues: 1-289; Coverage: 100%)
Gene names: TON_0896, csm4
CRISPR system Cms protein Csm5 Chain: F
Molecule details ›
Chain: F
Length: 397 amino acids
Theoretical weight: 45.26 KDa
Source organism: Thermococcus onnurineus NA1
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YWC2 (Residues: 1-397; Coverage: 100%)
Gene name: TON_0897
Sequence domains: RAMP superfamily
RNA (5'-R(*GP*UP*GP*GP*AP*AP*AP*GP*UP*GP*GP*CP*CP*CP*GP*AP*AP*AP*CP*CP*CP*UP*UP*C)-3') Chain: I
Molecule details ›
Chain: I
Length: 24 nucleotides
Theoretical weight: 7.71 KDa

Ligands and Environments

1 bound ligand:
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.35Å
Relevant EMDB volumes: EMD-9708
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins.
Jia et al. (2021)
1 more

2 mentions without citation

Cryo-EM structure of Type III-A CRISPR effector complex.
Huo et al. (2018)
1 more