› 6iu9

X-ray diffraction
3Å resolution

Crystal structure of cytoplasmic metal binding domain with iron ions

Released:
DOI: 10.2210/pdb6iu9/pdb
PDB entry 6iu9 coloured by chain, front view.
PDB entry 6iu9 coloured by chain, side view.
PDB entry 6iu9 coloured by chain, top view.
Source organism: Eucalyptus grandis
Primary publication:
Crystal structure of plant vacuolar iron transporter VIT1.
Kato T, Kumazaki K, Wada M, Taniguchi R, Nakane T, Yamashita K, Hirata K, Ishitani R, Ito K, Nishizawa T, Nureki O
Nat Plants 5 308-315 (2019)
PMID: 30742036

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VIT1 Chains: A, B, C, D, E, F, H, I
Molecule details ›
Chains: A, B, C, D, E, F, H, I
Length: 79 amino acids
Theoretical weight: 9.11 KDa
Source organism: Eucalyptus grandis
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ZN 20 x ZN
Ligand FE2 7 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P31
Unit cell:
a: 85.321Å b: 85.321Å c: 97.573Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.265
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi.
Sorribes-Dauden et al. (2020)
3 more

1 mention without citation

Characterization of the substrate binding site of an iron detoxifying membrane transporter from Plasmodium falciparum.
Sharma et al. (2021)