› 6ivm

X-ray diffraction
2.95Å resolution

Crystal structure of a membrane protein P143A

Released:
DOI: 10.2210/pdb6ivm/pdb
PDB entry 6ivm coloured by chain, front view.
PDB entry 6ivm coloured by chain, side view.
PDB entry 6ivm coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Dual Ca2+-dependent gates in human Bestrophin1 underlie disease-causing mechanisms of gain-of-function mutations.
Ji C, Kittredge A, Hopiavuori A, Ward N, Chen S, Fukuda Y, Zhang Y, Yang T
OpenAccess logo Commun Biol 2 240 (2019)
PMID: 31263784

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ibestrophin Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 297 amino acids
Theoretical weight: 33.81 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: W9BH30 (Residues: 1-294; Coverage: 96%)
Gene names: AI2879V1_1261, APT76_10705, B5L96_30355, BANRA_02438, BL124_00003590, DM078_14870, DM087_23365, DMR11_17555, DW281_00595, EAO17_00845, EAO28_28340, FXN67_01795, GJJ08_012945, GJJ13_012005, GJJ18_05220, GJJ21_05685, GNF00_19285, IE995_19765, NCTC11679_02573, NCTC13465_00112, NCTC5052_01714, NCTC8849_03195, NCTC9637_03467, NCTC9645_05950, NCTC9661_03571, SAMEA3499874_04755, SAMEA3512100_00236, SAMEA3531867_01148, SAMEA3538424_00427, SAMEA3538658_04281, SAMEA3649591_04364, SAMEA3649615_00575, SAMEA4370542_00410, SAMEA4873597_00862, VKR_02425, yneE
Sequence domains: Bestrophin, RFP-TM, chloride channel

Ligands and Environments

7 bound ligands:
Ligand ZN 17 x ZN
Ligand CL 12 x CL
Ligand EDO 1 x EDO
Ligand LEU 1 x LEU
Ligand ACY 2 x ACY
Ligand PG4 1 x PG4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 113.965Å b: 158.808Å c: 161.682Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structure and Function of the Bestrophin family of calcium-activated chloride channels.
Owji et al. (2021)
11 other articles