6j0n

Electron Microscopy
3.5Å resolution

Cryo-EM Structure of an Extracellular Contractile Injection System, baseplate in extended state, refined in C6 symmetry

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 54-mer (preferred)
PDBe Complex ID:
PDB-CPX-110393 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
IraD/Gp25-like domain-containing protein Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 140 amino acids
Theoretical weight: 15.99 KDa
Source organism: Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949
Expression system: Escherichia coli
UniProt:
  • Canonical: B6VNN6 (Residues: 1-140; Coverage: 100%)
Gene names: PA-RVA20-21-0163, PAU_03345
Sequence domains: Baseplate wedge protein gp25
Baseplate protein J-like domain-containing protein Chains: J, K, L, M, N, O
Molecule details ›
Chains: J, K, L, M, N, O
Length: 728 amino acids
Theoretical weight: 82.81 KDa
Source organism: Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949
Expression system: Escherichia coli
UniProt:
  • Canonical: B6VNN4 (Residues: 1-728; Coverage: 100%)
Gene names: PA-RVA20-21-0161, PAU_03343
Baseplate protein J-like domain-containing protein Chains: P, Q, R, S, T, U
Tail sheath protein C-terminal domain-containing protein Chains: V, W, X, Y, Z, a
Contractile injection system tube protein N-terminal domain-containing protein Chains: b, c, d, e, f, g
Phage tail sheath protein Chains: h, i, j, k, l, m
Phage tail protein Chains: n, o, p, q, r, s
Conserved hypothetical phage tail region protein Chains: 1, 2, 3, 4, 5, z
Phage tail sheath protein Chains: t, u, v, w, x, y

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-9765
Expression system: Escherichia coli