› 6j1i

X-ray diffraction
2.29Å resolution

Crystal structure of HypX from Aquifex aeolicus, A392F-I419F variant

Released:
DOI: 10.2210/pdb6j1i/pdb
PDB entry 6j1i coloured by chain, front view.
PDB entry 6j1i coloured by chain, side view.
PDB entry 6j1i coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural characterization of HypX responsible for CO biosynthesis in the maturation of NiFe-hydrogenase.
Muraki N, Ishii K, Uchiyama S, Itoh SG, Okumura H, Aono S
OpenAccess logo Commun Biol 2 385 (2019)
PMID: 31646188

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase regulation HoxX Chain: A
Molecule details ›
Chain: A
Length: 582 amino acids
Theoretical weight: 67.88 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O67224 (Residues: 1-562; Coverage: 100%)
Gene names: aq_1156, hoxX
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand COA 1 x COA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I222
Unit cell:
a: 72.701Å b: 139.639Å c: 167.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biophysical research in Okazaki, Japan.
Akiyama et al. (2020)
2 other articles

1 mention without citation

Structural characterization of HypX responsible for CO biosynthesis in the maturation of NiFe-hydrogenase.
Muraki et al. (2019)